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This happened to me on Sunday evening. Although I was on the windows 32bit client. I initiated a trade with a fellow group member in cyro and couldn't split an item stack. On cancelling out and checking my inventory, I couldn't split a stack there either.
To clarify, the option was not present in the menu on right clicking on the item.
On checking there now, I do indeed have the option. I believe this to be a random occurrence rather than a situational / permanent one.
350m+ AP PC - EU
AD :: Imported Waffles [37]EP :: Wee ee ee ee ee [16]DC :: Ghostbane's DK [16], Impending Loadscreen [12]PC - NA
AD :: Ghostbane [50], yer ma [43], Sir Humphrey Winterbottom 2.0 [18], robotic baby legs [18]EP :: Wee Mad Arthur [50], avast ye buttcrackz [49], Sir Horace Foghorn [27], Brother Ballbag [24], Scatman John [16]DC :: W T B Waffles [36], Morale Boost [30], W T F Waffles [17], Ghostbanë [15]RIPAD :: Sir Humphrey Winterbottom 1.0 [20]
I just logged into the game and now I can split stacks again. Very odd random bug. BTW, thanks for the suggestion but I did have bag space available. I had 2 bag slots left yesterday, I went and bought 2 bag upgrades for the toon that couldn't split stacks and it didn't help. Seems fine this morning though.